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Determination of doxazosin and verapamil in human serum by fast LC-MS/MS: Application to document non-compliance of patients
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SYSNO ASEP 0352169 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Determination of doxazosin and verapamil in human serum by fast LC-MS/MS: Application to document non-compliance of patients Author(s) Chytil, L. (CZ)
Štrauch, B. (CZ)
Cvačka, Josef (UOCHB-X) RID, ORCID
Marešová, V. (CZ)
Widimský jr., J. (CZ)
Holaj, R. (CZ)
Slanař, O. (CZ)Number of authors 7 Source Title Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 878, č. 30 (2010), s. 3167-3173Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords doxazosin ; verapamil ; hypertension Subject RIV CC - Organic Chemistry CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000284672300018 DOI 10.1016/j.jchromb.2010.09.032 Annotation A rapid and sensitive method using liquid chromatography–tandem mass spectrometry for simultaneous determination of doxazosin and verapamil in human serum has been developed. Trimipramine-d3 as an isotopic labelled internal standard was used for quantification. Serum samples were prepared by simple liquid–liquid extraction with mixture of tert butyl methyl ether and ethyl acetate. The analytes and internal standard were separated on C18 column using an isocratic elution. Positive TurboIonSpray mass spectrometry was used with multiple reaction monitoring of the transitions. An extensive pre-study method validation was carried out in accordance with FDA guidelines. This assay was successfully applied to determine the serum concentrations of doxazosin and verapamil in suspect non-compliance patients. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2011
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