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Role of FtsH2 in the repair of Photosystem II in mutants of the cyanobacterium Synechocystis PCC 6803 with impaired assembly or stability of the CaMn4 cluster
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SYSNO ASEP 0351624 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Role of FtsH2 in the repair of Photosystem II in mutants of the cyanobacterium Synechocystis PCC 6803 with impaired assembly or stability of the CaMn4 cluster Author(s) Komenda, Josef (MBU-M) RID, ORCID
Knoppová, Jana (MBU-M) RID
Krynická, Vendula (MBU-M) RID
Nixon, P. J. (GB)
Tichý, Martin (MBU-M) RIDSource Title Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1797, č. 5 (2010), s. 566-575Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords CtpA protease ; D1 degradation and maturation ; FtsH protease Subject RIV EE - Microbiology, Virology R&D Projects IAA400200801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000276621100006 DOI 10.1016/j.bbabio.2010.02.006 Annotation The FtsH2 protease, encoded by the slr0228 gene, plays a key role in the selective degradation of photodamaged D1 protein during the repair of Photosystem II (PSII) in the cyanobacterium Synechocystis sp. PCC 6803. To test whether additional proteases might be involved in D1 degradation during high rates of photodamage, we have studied the synthesis and degradation of the D1 protein in DPsbO and DPsbV mutants, in which the CaMn4 cluster catalyzing oxygen evolution is less stable, and in the D1 processing mutants, D1-S345P and DCtpA, which are unable to assemble a functional cluster. All four mutants exhibited a dramatically increased rate of D1 degradation in high light compared to the wild type. Additional inactivation of the ftsH2 gene slowed the rate of D1 degradation dramatically and increased the level of PSII complexes. We conclude that FtsH2 plays a major role in the degradation of both precursor and mature forms of D1 following donor-side photoinhibition Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2011
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