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A dual surface plasmon resonance assay for the determination of ribonuclease H activity
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SYSNO ASEP 0350621 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A dual surface plasmon resonance assay for the determination of ribonuclease H activity Author(s) Šípová, Hana (URE-Y)
Vaisocherová, Hana (URE-Y) RID
Štepánek, J. (CZ)
Homola, Jiří (URE-Y) RIDNumber of authors 4 Source Title Biosensors and Bioelectronics. - : Elsevier - ISSN 0956-5663
Roč. 26, č. 4 (2010), s. 1605-1611Number of pages 7 s. Language eng - English Country GB - United Kingdom Keywords Surface plasmon resonance ; Enzyme activity assay ; Ribonuclease H ; Biosensor Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) OC09058 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA202/09/0193 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z20670512 - URE-Y (2005-2011) UT WOS 000286403400071 DOI 10.1016/j.bios.2010.08.011 Annotation We report on a new assay based on surface plasmon resonance (SPR) which allows for simultaneous 1) real-time monitoring of RNase H activity and 2) detection of cleavage reaction products. The potential of the assay is demonstrated in the study in which the ability of natural and modified oligonucleotides to activate RNase H is examined. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2011
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