Specific cell cultivation on nanofibrous layer

Michálek, Jiří; Mareková, Dana; Martinová, L.; Hobzová, Radka; Kostecká, Petra; Lubasová, D.; Lesný, Petr; Přádný, Martin; Jendelová, Pavla; Syková, Eva

doi:https://dx.doi.org/10.4028/www.scientific.net/AST.76.139

Number of the records: 1

Specific cell cultivation on nanofibrous layer

1.

SYSNO ASEP	0350418
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Specific cell cultivation on nanofibrous layer
Author(s)	Michálek, Jiří (UMCH-V)_{RID, ORCID} Mareková, Dana (UEM-P) Martinová, L. (CZ) Hobzová, Radka (UMCH-V)_{RID, ORCID} Kostecká, Petra (UEM-P) Lubasová, D. (CZ) Lesný, Petr (UEM-P) Přádný, Martin (UMCH-V)_RID Jendelová, Pavla (UEM-P)_{RID, ORCID} Syková, Eva (UEM-P)_RID
Source Title	Advances in Science and Technology - ISSN 1662-0356 Roč. 76, - (2010), s. 139-142
Number of pages	4 s.
Action	International Conferences on Modern Materials & Technologies, Forum on New Materials /5./
Event date	13.06.2010-18.06.2010
VEvent location	Montecatini Terme
Country	IT - Italy
Event type	WRD
Language	eng - English
Country	CH - Switzerland
Keywords	hepatocytes ; hepatal bioreactor ; bioartifical liver assist device
Subject RIV	CD - Macromolecular Chemistry
R&D Projects	GA304/07/1129 GA ČR - Czech Science Foundation (CSF)
	KJB400500902 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z40500505 - UMCH-V (2005-2011)
	AV0Z50390703 - UEM-P (2007-2013)
DOI	10.4028/www.scientific.net/AST.76.139
Annotation	Nanofibrous layers of reproducible thickness and structure (Nanospider™ technology) were employed as carriers for hepatocytes and their efficiency were evaluated in a laboratory bioreactor. The construction of the functional hepatal bioreactor is particularly given by the positive response of hepatocyte cells to the used carrier layer as well as by the cell morphology, their viability and biological activity in certain period of time. We compared cell growth on collagen with nanofibres electrospun from copolymer (HEMA/EOEMA) and from differently prepared polycaprolactone (PCL) layers. In both cases, the cells showed better morphology or better growth the on controls (collagen). The results obtained in laboratory bioreactor proved the biochemical functionality of the studied system.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2011

Number of the records: 1