Arabidopsis RAB geranylgeranyl transferase beta-subunit mutant is constitutively photomorphogenic, and has shoot growth and gravitropic defects

Hála, Michal; Soukupová, Hana; Synek, Lukáš; Žárský, Viktor

doi:https://dx.doi.org/10.1111/j.1365-313X.2010.04172.x

Number of the records: 1

Arabidopsis RAB geranylgeranyl transferase beta-subunit mutant is constitutively photomorphogenic, and has shoot growth and gravitropic defects

1.

SYSNO ASEP	0350388
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Arabidopsis RAB geranylgeranyl transferase beta-subunit mutant is constitutively photomorphogenic, and has shoot growth and gravitropic defects
Author(s)	Hála, Michal (UEB-Q)_{RID, ORCID} Soukupová, Hana (UEB-Q)_{RID, ORCID} Synek, Lukáš (UEB-Q)_{RID, ORCID} Žárský, Viktor (UEB-Q)_{RID, ORCID}
Source Title	Plant Journal. - : Wiley - ISSN 0960-7412 Roč. 62, č. 4 (2010), s. 615-627
Number of pages	13 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	RAB GTPases ; cell expansion ; photomorphogenesis
Subject RIV	EA - Cell Biology
R&D Projects	GP204/06/P467 GA ČR - Czech Science Foundation (CSF)
	KJB600380802 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000277609500007
DOI	10.1111/j.1365-313X.2010.04172.x
Annotation	RAB GTPases are key regulators of intracellular vesicle transport. They are modified by geranylgeranylation catalyzed by the RAB geranylgeranyl transferase (RAB-GGT). Here, we show that RAB-GGT activity is significantly reduced in two homozygous mutants of the major Arabidopsis beta-subunit of RAB-GGT (AtRGTB1), resulting in unprenylated RAB GTPases accumulation in the cytoplasm. Both endocytosis and exocytosis are downregulated in rgtb1 homozygotes defective in shoot growth and morphogenesis. Root gravitropism is normal in rgtb1 roots, but is significantly compromised in shoots. Mutants are defective in etiolation and show constitutive photomorphogenic phenotypes that cannot be rescued by brassinosteroid treatment, similarly to the secretory det3 mutant. Transcriptomic analysis revealed an upregulation of specific RAB GTPases in etiolated wild-type plants. These data suggest that the downregulation of the secretory pathway is interpreted as a photomorphogenic signal in Arabidopsis.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1