CELL STRUCTURAL REORGANIZATION DURING INDUCTION OF ANDROGENESIS IN ISOLATED MICROSPORE CULTURES OF TRITICALE (x TRITICOSECALE WITTM.)

0350381 - ÚEB 2011 RIV PL eng J - Journal Article
Dubas, E. - Wedzony, M. - Petrovská, Beáta - Salaj, J. - Zur, I.
CELL STRUCTURAL REORGANIZATION DURING INDUCTION OF ANDROGENESIS IN ISOLATED MICROSPORE CULTURES OF TRITICALE (x TRITICOSECALE WITTM.).
Acta Biologica Cracoviensia Series Botanica. Roč. 52, č. 1 (2010), s. 73-86. ISSN 0001-5296. E-ISSN 1898-0295
Institutional research plan: CEZ:AV0Z50380511
Keywords : Androgenesis * microspores * triticale
Subject RIV: EF - Botanics
Impact factor: 0.586, year: 2010
www.ib.uj.edu.pl/abc/pdf/52_1/10_dubas.pdf http://arl-repository.lib.cas.cz/uloziste_av/UEB-Q/cav_un_epca-0350381_01.pdf

Upon stress treatment isolated microspores of triticale were directed towards sporophytic development.We used fluorescence microscopy to study the cell structural reorganization associated with the process Changes in the developmental pathway coincided with the character of the microtubular cytoskeleton configuration. the number and direction of nuclear divisions, changes in vacuolization, the dtstribution of mitochondria. ER and starch grains, and the architecture of new cell wall formation. A band of diffused fluorescence surrounding the nucleus was observed before the first symmetric division of microspores This structure most likely represents a preprophase band (PPB). Successive mitotic divisions within the microspore wall led to the formation of multinucleate or multicellular structures consisting of one or two domains of cells differing in size. They were later released from the sporoderm and continued further development with features typical for a monocotyledonous embryo.
Permanent Link: http://hdl.handle.net/11104/0190398