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Sustainable use of Genetic Diversity in Forage and Turf Breeding
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SYSNO ASEP 0349995 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title DArTFest – A Platform for High-Throughput Genome Profiling Within the Festuca – Lolium Complex Author(s) Kopecký, David (UEB-Q) RID, ORCID
Bartoš, Jan (UEB-Q) RID, ORCID
Lukaszewski, A.J. (US)
Baird, J. H. (US)
Černoch, V. (CZ)
Kölliker, R. (NL)
Sandve, S.R. (NO)
Rognli, O.A. (DK)
Blois, H. (AU)
Caig, V. (AU)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Kilian, A. (AU)Source Title Sustainable use of Genetic Diversity in Forage and Turf Breeding. - Dordrecht : Springer, 2010 / Huyghe Ch. - ISBN 978-90-481-8705-8 Pages s. 443-448 Number of pages 6 s. Number of copy 700 Number of pages 572 Language eng - English Country NL - Netherlands Keywords DArT ; Fescue ; Hybrids Subject RIV EB - Genetics ; Molecular Biology R&D Projects QH71267 GA MZe - Ministry of Agriculture (MZe) GP521/07/P479 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) DOI 10.1007/978-90-481-8706-5_65 Annotation With the aim to facilitate high-throughput genome profiling and genetic and physical mapping within the Festuca-Lolium, complex we have developed a Diversity Arrays Technology (DArT) array for five important species: Festuca pratensis, Festuca arundinacea, Festuca glaucescens, Inlium perenne and Lolium multiflorum. The DArTFest array contains 7,680 probes derived from methylfiltered genomic representations. Of 3,884 polymorphic DArT markers identified in the first marker discovery experiment, over 1,000 markers detected a positive allele in each species. We assigned DArT markers to individual chromosome regions of F. pratensis using a series of single chromosome substitution and recombinant lines of F. pratensis in L. multiflorum. Moreover, we enriched the existing genetic map of F. pratensis by over 200 DArT markers and existing genetic map of L. multiflorum by over 500 DART markers. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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