Isolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps

Wenzl, P.; Suchánková, Pavla; Carling, J.; Šimková, Hana; Huttner, E.; Kubaláková, Marie; Sourdille, P.; Paul, E.; Feuillet, C.; Kilian, A.; Doležel, Jaroslav

doi:https://dx.doi.org/10.1007/s00122-010-1323-8

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Isolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps

1.

SYSNO ASEP	0349830
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Isolated chromosomes as a new and efficient source of DArT markers for the saturation of genetic maps
Author(s)	Wenzl, P. (MX) Suchánková, Pavla (UEB-Q)_RID Carling, J. (AU) Šimková, Hana (UEB-Q)_{RID, ORCID} Huttner, E. (AU) Kubaláková, Marie (UEB-Q)_RID Sourdille, P. (FR) Paul, E. (US) Feuillet, C. (FR) Kilian, A. (AU) Doležel, Jaroslav (UEB-Q)_{RID, ORCID}
Source Title	Theoretical and Applied Genetics. - : Springer - ISSN 0040-5752 Roč. 121, č. 3 (2010), s. 465-474
Number of pages	10 s.
Language	eng - English
Country	DE - Germany
Keywords	WHEAT TRITICUM-AESTIVUM ; ARRAYS TECHNOLOGY DART ; HEXAPLOID WHEAT
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000280090700005
DOI	10.1007/s00122-010-1323-8
Annotation	We describe how the diversity arrays technology (DArT) can be coupled with chromosome sorting to increase the density of genetic maps in specific genome regions. Chromosome 3B and the short arm of chromosome 1B (1BS) of wheat were isolated by flow cytometric sorting and used to develop chromosome- and chromosome arm-enriched genotyping arrays containing 2,688 3B clones and 384 1BS clones. Linkage analysis showed that 553 of the 711 polymorphic 3B-derived markers (78%) mapped to chromosome 3B, and 59 of the 68 polymorphic 1BS-derived markers (87%) mapped to chromosome 1BS, confirming the efficiency of the chromosome-sorting approach. To demonstrate the potential for saturation of genetic maps, we constructed a consensus map of chromosome 3B using 19 mapping populations, including some that were genotyped with the 3B-enriched array. The 3B-derived DArT markers doubled the number of genetic loci covered.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2011

Number of the records: 1