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Insights into the effect of soil pH on N.sub.2./sub.O and N.sub.2./sub. emissions and denitrifier community size and activity
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SYSNO ASEP 0349812 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Insights into the effect of soil pH on N2O and N2 emissions and denitrifier community size and activity Author(s) Čuhel, Jiří (BC-A)
Šimek, Miloslav (BC-A) RID, ORCID
Laughlin, R.J. (GB)
Bru, D. (FR)
Chéneby, D. (FR)
Watson, C.J. (GB)
Philippot, L. (FR)Source Title Applied and Environmental Microbiology. - : American Society for Microbiology - ISSN 0099-2240
Roč. 76, č. 6 (2010), s. 1870-1878Number of pages 9 s. Language eng - English Country US - United States Keywords soil pH ; N2O and N2 emissions ; denitrifier community Subject RIV EH - Ecology, Behaviour R&D Projects MEB020726 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06066 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA600660605 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z60660521 - UPB-H, BC-A (2005-2011) UT WOS 000275193900019 DOI 10.1128/AEM.02484-09 Annotation The objective of this study was to investigate how changes in soil pH affect the N2O and N2 emissions, denitrification activity, and size of a denitrifier community. We established a field experiment, situated in a grassland area, which consisted of three treatments which were repeatedly amended with a KOH solution (alkaline soil), an H2SO4 solution (acidic soil), or water (natural pH soil) over 10 months. At the site, we determined field N2O and N2 emissions using the 15N gas flux method and collected soil samples for the measurement of potential denitrification activity and quantification of the size of the denitrifying community by quantitative PCR of the narG, napA, nirS, nirK, nosZ denitrification genes. Overall, our results indicate that soil pH is of importance in determining the nature of denitrification end products. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
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