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Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing
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SYSNO ASEP 0349182 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing Author(s) Wu, S.W. (CN)
Xiao, Y. (CN)
Zheng, X. (CN)
Cai, Y.F. (CN)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Liu, B.H. (CN)
Yang, L. (CN)
Song, M.F. (CN)
Zhou, P. (CN)
Zhou, Y. (CN)
Meng, F.H. (CN)
Wang, S.H. (CN)
Liu, H.W. (CN)
Zhai, H.Q. (CN)
Yang, J.P. (CN)Source Title Chinese Science Bulletin - ISSN 1001-6538
Roč. 55, č. 15 (2010), s. 1463-1468Number of pages 6 s. Language eng - English Country CN - China Keywords Triticum aestivum ; flow cytogenetics ; chromosome sorting Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000278704100001 DOI 10.1007/s11434-010-3106-7 Annotation The large genome size (similar to 17000 Mb) and complicated DNA structures of common wheat (Triticum aestivum) hamper its genome sequencing. By means of flow cytometry, systematic investigations on individual chromosome sorting have been carried out to construct chromosome-specific bacterial artificial chromosome (BAC) libraries since the 1980s. Several wheat chromosome-specific BAC libraries, such as chromosome 3B, three D genome chromosomes (1D, 4D and 6D), and the short arm of chromosome 1B, have been developed, and the physical map of chromosome 3B was established in 2008. The same chromosome-based strategy is being employed by the International Wheat Genome Sequencing Consortium (IWGSC) to establish the physical maps of the other 20 common wheat chromosomes (cv. Chinese Spring). Several projects on wheat genome sequencing are currently underway. The availability of new sequencing technologies provides new choices for sequencing of gene space of the wheat genome. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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