Number of the records: 1
Multi-component polymeric system for tumour cell-specific gene delivery using a universal bungarotoxin linker
- 1.
SYSNO ASEP 0349126 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Multi-component polymeric system for tumour cell-specific gene delivery using a universal bungarotoxin linker Author(s) Willemsen, R. A. (NL)
Pechar, Michal (UMCH-V) RID, ORCID
Carlisle, R. C. (GB)
Schooten, E. (NL)
Pola, Robert (UMCH-V) RID, ORCID
Thompson, A. J. (GB)
Seymour, L. W. (GB)
Ulbrich, Karel (UMCH-V) RIDSource Title Pharmaceutical Research. - : Springer - ISSN 0724-8741
Roč. 27, č. 11 (2010), s. 2274-2282Number of pages 9 s. Language eng - English Country US - United States Keywords adenovirus ; hydrophilic polymers ; tumour targeting Subject RIV CD - Macromolecular Chemistry R&D Projects GA203/08/0543 GA ČR - Czech Science Foundation (CSF) KAN200200651 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000283368000003 DOI 10.1007/s11095-010-0088-8 Annotation A new universal tool for specific, non-covalent and non-destructive attachment of a recombinant antibody fragment to a polymer-modified adenovirus has been utilised to regulate the tropism of adenoviral gene delivery vector. We have prepared a multivalent reactive N-(2-hydroxypropyl)methacrylamide-based copolymer (PHPMA) bearing an alpha-bungarotoxin-binding peptide (BTXbp). The copolymer was used for covalent surface modification of adenoviral vectors (Ad). A single chain variable fragment of anti-PSMA antibody bearing bungarotoxin (scFv-BTX) binding to the prostate-specific membrane antigen (PSMA) was conjugated with the copolymer-coated Ad. The presented Ad-PHPMA-BTXbp/scFv-BTX system can be used as a universal tool for a receptor-specific virotherapy. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2011
Number of the records: 1