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2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing
- 1.0348081 - ÚIACH 2011 RIV NL eng J - Journal Article
Petry-Podgórska, Inga - Žídková, Jitka - Flodrová, Dana - Bobálová, Janette
2D-HPLC and MALDI-TOF/TOF analysis of barley proteins glycated during brewing.
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. Roč. 878, č. 30 (2010), s. 3143-3148. ISSN 1570-0232. E-ISSN 1873-376X
R&D Projects: GA MŠMT 1M0570
Institutional research plan: CEZ:AV0Z40310501
Keywords : 2D-HPLC * MALDI-TOF/TOF mass spectrometry * barley
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.971, year: 2010
A combination of two-dimensional high performance liquid chromatography (2D-HPLC) and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/TOF MS) was used for the analysis of the protein extracts that were reduced, alkylated, and degraded enzymatically without prior protein separation. This so-called “shot-gun” approach enabled us to determine glycation sites in one third of the proteins identified in the study and to propose potential glycation markers for fast and efficient monitoring during malting.
Permanent Link: http://hdl.handle.net/11104/0188698
Number of the records: 1