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Cytokinin receptor antagonists derived from 6-benzylaminopurine
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SYSNO ASEP 0348001 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cytokinin receptor antagonists derived from 6-benzylaminopurine Author(s) Nisler, Jaroslav (UEB-Q) RID, ORCID
Zatloukal, Marek (UEB-Q) ORCID
Popa, Igor (UEB-Q)
Doležal, Karel (UEB-Q) RID, ORCID
Strnad, Miroslav (UEB-Q) RID, ORCID
Spíchal, Lukáš (UEB-Q) RID, ORCIDSource Title Phytochemistry. - : Elsevier - ISSN 0031-9422
Roč. 71, č. 7 (2010), s. 823-830Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords Cytokinin ; Anticytokinin ; Cytokinin receptor Subject RIV CE - Biochemistry R&D Projects GD522/08/H003 GA ČR - Czech Science Foundation (CSF) GP522/07/P197 GA ČR - Czech Science Foundation (CSF) LC06034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000277908400016 DOI 10.1016/j.phytochem.2010.01.018 Annotation Recently we reported 6-(2-hydroxy-3-methylbenzylamino)purine (PI-55) as the first molecule to antagonize cytokinin activity at the receptor level. Here we report the synthesis and in vitro biological testing of eleven BAP derivatives substituted in the benzyl ring and in the C2, N7 and N9 positions of the purine moiety. The ability of the compounds to interact with Arabidopsis cytokinin receptors AHK3 and CRE1/AHK4 was tested in bacterial receptor and in live-cell binding assays, and in an Arabidopsis ARR5:GUS (Arabidopsis response regulator 5) reporter gene assay. Cytokinin activity of the compounds was determined in classical cytokinin biotests (tobacco callus, wheat leaf senescence and Amaranthus bioassays). 6(2,5-Dihydroxybenzylamino)purine (LGR-991) was identified as a cytokinin receptor antagonist. At the molecular level LGR-991 blocks the cytokinin receptor CRE1/AHK4 with the same potency as PI-55. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2011
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