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Diversity of Anaerobic Fungi within Cow Manure Determined by ITS1 Analysis
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SYSNO ASEP 0347929 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Diversity of Anaerobic Fungi within Cow Manure Determined by ITS1 Analysis Author(s) Fliegerová, Kateřina (UZFG-Y) RID, ORCID
Mrázek, Jakub (UZFG-Y) RID, ORCID
Hoffmann, K. (DE)
Zábranská, J. (CZ)
Voigt, K. (DE)Source Title Folia Microbiologica. - : Springer - ISSN 0015-5632
Roč. 55, č. 4 (2010), s. 319-325Number of pages 6 s. Language eng - English Country CZ - Czech Republic Keywords GUT FUNGI ; RUMEN ; IDENTIFICATION Subject RIV EH - Ecology, Behaviour R&D Projects GPP503/10/P394 GA ČR - Czech Science Foundation (CSF) QI92A286 GA MZe - Ministry of Agriculture (MZe) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000281277900004 Annotation The diversity of anaerobic fungi was evaluated in cow semiliquid manure obtained from input homogenizing tank of biogas plant. Among three sets of tested primers, the combination of fungal universal ITS1F and Neocallimastigales specific Neo QPCR Rev primers was selected and used for the construction of clone library. Eighty-four new complete internal transcribed spacers (ITS1) and partial 5.8S rDNA sequences generated within this study were analyzed by Bayesian inference and assigned to an existing order of the Neocallimastigales. Sixty-seven % of sequences were affiliated with Cyllamyces, 24 % with Piromyces, 7 % with Anaeromyces, only 2 % with Neocallimastix, and no sequences with Orpinomyces. According to Bayesian analysis the genus Caecomyces was polyphyletic and disappeared from the presented ITS-based phylogram. This study gave a first insight into the diversity of anaerobic fungi in cow manure, where the prevalence of fungi with bulbous morphology was indicated. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2011
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