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YCF45 protein, usually associated with plastids, is targeted into the mitochondrion of Trypanosoma brucei
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SYSNO ASEP 0347288 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title YCF45 protein, usually associated with plastids, is targeted into the mitochondrion of Trypanosoma brucei Author(s) Týč, Jiří (BC-A) ORCID, RID
Long, Shaojun (BC-A)
Jirků, Milan (BC-A) RID
Lukeš, Julius (BC-A) RID, ORCIDSource Title Molecular and Biochemical Parasitology. - : Elsevier - ISSN 0166-6851
Roč. 173, č. 1 (2010), s. 43-47Number of pages 5 s. Language eng - English Country NL - Netherlands Keywords Trypanosoma ; Plastid ; Mitochondrion ; Targeting ; YCF45 ; Horizontal gene transfer Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/09/1667 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000279706900007 DOI 10.1016/j.molbiopara.2010.05.002 Annotation YCF45 belongs to a family of proteins of unknown function usually located in the chloroplast of plants. Its highly conserved homologues were found in the genomes of several Trypanosoma and Leishmania species. HA(3)-tagging of the YCF45 protein with the start codon as annotated in the Gene(DB) revealed its cytosolic localization in the cultured procyclic stage of Trypanosoma brucei. However, when a more upstream located start codon was used in another HA(3)-tagged construct, the resulting protein was targeted to the mitochondrion. We propose that YCF45 was acquired by an ancestral trypanosomatid by horizontal gene transfer and in the absence of a plastid was re-targeted to the mitochondrion. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
Number of the records: 1