Synthesis of unsymmetric cyanine dye via merocyanine and their interaction with DNA

0347152 - ÚMG 2011 RIV CZ eng J - Journal Article
Bříza, T. - Kejík, Z. - Králová, Jarmila - Martásek, P. - Král, V.
Synthesis of unsymmetric cyanine dye via merocyanine and their interaction with DNA.
Collection of Czechoslovak Chemical Communications. Roč. 78, 7-8 (2009), s. 1081-1090. ISSN 0010-0765
Institutional research plan: CEZ:AV0Z50520514
Keywords : malondialdehyde * merocyanine * DNA
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.856, year: 2009

An effective method has been developed for synthesis of neutral merocyanine dye 4 from (4-nitrophenyl) malondialdehyde. Merocyanine 4 was used as a basic building block for preparation of unsymmetric cyanine dyes 9 and 10, both derived from the same aromatic malondialdehyde. With the cyanine 10, pH-dependent affinity to DNA has been studied. Unsymmetric cyanine dye 10 has been compared with symmetric dye 8. The corresponding binding constants (K) have been calculated for various pH.
Permanent Link: http://hdl.handle.net/11104/0187992