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RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription
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SYSNO ASEP 0347094 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title RECQ5 helicase associates with the C-terminal repeat domain of RNA polymerase II during productive elongation phase of transcription Author(s) Kanagaraj, R. (CH)
Huehn, D. (CH)
Mackellar, A. (US)
Menigatti, M. (CH)
Zheng, L. (CH)
Urban, Václav (UMG-J)
Shevelev, Igor (UMG-J)
Greenleaf, A.L. (US)
Janščák, Pavel (UMG-J) RIDSource Title Nucleic Acids Research. - : Oxford University Press - ISSN 0305-1048
Roč. 38, č. 22 (2010), s. 8131-8140Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords RECQ5 DNA helicase ; transcription ; genome stability Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/09/0565 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000285420300031 DOI 10.1093/nar/gkq697 Annotation It is known that transcription can induce DNA recombination, thus compromising genomic stability. RECQ5 DNA helicase promotes genomic stability by regulating homologous recombination. Recent studies have shown that RECQ5 forms a stable complex with RNA polymerase II (RNAPII) in human cells, but the cellular role of this association is not understood. Here, we provide evidence that RECQ5 specifically binds to the Ser2,5-phosphorylated C-terminal repeat domain (CTD) of the largest subunit of RNAPII, RPB1, by means of a Set2–Rpb1-interacting (SRI) motif located at the C-terminus of RECQ5. We also show that RECQ5 associates with RNAPII-transcribed genes in a manner dependent on the SRI motif. Notably, RECQ5 density on transcribed genes correlates with the density of Ser2-CTD phosphorylation, which is associated with the productive elongation phase of transcription. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2011
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