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Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices
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SYSNO ASEP 0346926 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices Author(s) Coll, A. (ES)
Nadal, A. (ES)
Collado, R. (ES)
Capellades, G. (ES)
Kubista, Mikael (BTO-N) RID
Messeguer, J. (ES)
Pla, M. (ES)Source Title Plant Molecular Biology. - : Springer - ISSN 0167-4412
Roč. 73, č. 3 (2010), s. 349-362Number of pages 13 s. Language eng - English Country US - United States Keywords GMO (Genetically Modified Organism) ; MON810 ; Maize ; Transcriptome Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000276911800009 DOI 10.1007/s11103-010-9624-5 Annotation Over the last few years, targeted approaches have been complemented by profiling methods to assess possible unintended effects of transformation of GMO. Here we used Affymetrix microarray platform to evaluate transcriptional differences between commercial MON810 GM maize and non-transgenic crops. We took two common MON810/non-GM variety pairs and two farming practices. MON810 and comparable non-GM varieties have very low numbers of sequences with differential expression. We show, the differences between a given MON810 variety and the non-GM do not depend on the assayed cultural conditions. Natural variation explained most of the variability in gene expression. Up to 37 % was dependent upon the variety and 32 % a result of the fertilization. The MON810 had minor effect (9.7%) on gene expression. This indicates that transcriptional differences of conventionally-bred varieties and different environmental conditions should be taken into account in safety assessment studies of GM plants. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2011
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