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Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry
- 1.0346664 - ÚMCH 2011 RIV GB eng J - Journal Article
Kuchař, L. - Rotková, J. - Asfaw, B. - Lenfeld, Jiří - Horák, Daniel - Korecká, L. - Bílková, Z. - Ledvinová, J.
Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry.
Rapid Communications in Mass Spectrometry. Roč. 24, č. 16 (2010), s. 2393-2399. ISSN 0951-4198. E-ISSN 1097-0231
R&D Projects: GA ČR GA203/09/0857
Institutional research plan: CEZ:AV0Z40500505
Keywords : reverse hydrolysis reaction * quantitative-determination * internal standards
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.846, year: 2010
Sphingolipid ceramide N-deacylase (SCDase, EC 3.5.1.69) is a hydrolytic enzyme isolated from Psendomonas sp. TK 4. In addition to its primary deacylation function, this enzyme is able to reacylate lyso-sphingolipids under specific conditions. We immobilized the enzyme on magnetic macroporous cellulose and used it to semisynthesise C17:0 glucosylceramide and C17:0 sulphatide.
Permanent Link: http://hdl.handle.net/11104/0005994
Number of the records: 1