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Antitumor activity od apoptotic nuclease TBN1 from L. esculentum
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SYSNO ASEP 0346342 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antitumor activity od apoptotic nuclease TBN1 from L. esculentum Author(s) Matoušek, Jaroslav (BC-A) RID, ORCID
Podzimek, Tomáš (BC-A)
Poučková, P. (CZ)
Stehlík, Jan (BC-A)
Škvor, J. (CZ)
Lipovová, P. (CZ)
Matoušek, Josef (UZFG-Y) RIDNumber of authors 7 Source Title Neoplasma - ISSN 0028-2685
Roč. 57, č. 4 (2010), s. 339-348Number of pages 10 s. Language eng - English Country SK - Slovakia Keywords anticancerogenic and antiproliferative nuclease ; dsRNase ; human solid malignant tumors Subject RIV FD - Oncology ; Hematology R&D Projects GA521/06/1149 GA ČR - Czech Science Foundation (CSF) GA521/09/1214 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000278262000008 DOI 10.4149/neo_2010_04_339 Annotation Nuclease from tomato (TBN1) wad produced by in planta biotechnology, purified and tested for its antitumor properties. The nuclease was cytostatic after its intratumoral administration to nude mice bearing human melanoma or prostate carcinoma, and also after tumor targeting by its intravenous administration as conjugate with PEG. Inhibitory effects of TBN1 on tumor growth were comparable to effects of bovine seminal RNase (BS-RNase), but the inhibition was reached at about ten times lower protein concentration. Intravenous administration of TBN1-PEG caused significant inhibition of tumor proliferation without obvious degenerative changes, while direct administration of TBN1 into melanoma tumors led to rapid tumor tissue degeneration. Simultaneously, TBN1 exhibited a lower degree of embryotoxicity compared to BS-RNase and other nucleases. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
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