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First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic
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SYSNO ASEP 0345901 Document Type O - Others R&D Document Type Others Title First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic Author(s) Přibylová, Jaroslava (BC-A) RID, ORCID
Špak, Josef (BC-A) RID, ORCID
Kubelková, Darina (BC-A)
Petrzik, Karel (BC-A) RID, ORCIDNumber of authors 4 Source Title Plant Disease. - : American Phytopathological Society - ISSN 0191-2917
Roč. 94, č. 8 (2010), s. 1071-1071Number of pages 1 s. Language eng - English Country US - United States Keywords Virus ; small fruits ; pathogen detection Subject RIV EE - Microbiology, Virology R&D Projects OC09022 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50510513 - UMBR-M, BC-A (2005-2011) UT WOS 000280058900045 DOI 10.1094/PDIS-94-8-1071B Annotation A collection of highbush blueberry (Vaccinium corymbosum L.) cultivars was surveyed in 2009. A total of 67 plants of 10 cultivars were observed for typical Blueberry red ringspot virus (BRRV) symptoms and were also assayed for BRRV infection using PCR. Extracted DNA from leaf tissue was used in PCR for BRRV detection with primer pair BRRV15/16. No BRRV symptoms were observed on the plants in early spring, yet BRRV was detected in one symptom-free bush of cv. Darrow by PCR. In July, typical symptoms developed on that and another cv. Darrow bush that was also positive by PCR. DNA fragments were amplified from total nucleic acid samples of both infected blueberry bushes using primers BRRV15/16. The amplicons were sequenced and revealed 97.5% nucleotide sequence identity to the BRRV (GenBank accession number AF404509). The 845 nucleotide sequence of the amplicon has been deposited at GenBank under accession number HM107773. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2013
Number of the records: 1