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Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy
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SYSNO ASEP 0345815 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy Author(s) Macháň, Radek (UFCH-W)
Hof, Martin (UFCH-W) RID, ORCIDSource Title Biochimica Et Biophysica Acta-Biomembranes. - : Elsevier - ISSN 0005-2736
Roč. 1798, č. 7 (2010), s. 1377-1391Number of pages 15 s. Language eng - English Country NL - Netherlands Keywords supported lipid bilayer ; giant unilamellar vesicle ; fluorescence recovery after photobleaching Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GA203/08/0114 GA ČR - Czech Science Foundation (CSF) GEMEM/09/E006 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000279365000012 DOI 10.1016/j.bbamem.2010.02.014 Annotation Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure: methods based on optical microscopy are very convenient for such investigations. We focus on fluorescence correlation spectroscopy (FCS), explain its principles and review its state of the art versions such as 2-focus. Z-scan or scanning FCS, which overcome most artefacts of standard FCS (especially those resulting from the need for an external calibration) making it a reliable and versatile method. FCS is also compared to single particle tracking and fluorescence photobleaching recovery and the applicability and the limitations of the methods are briefly reviewed. We discuss several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition (ionic strength and sugar content), choice of a fluorescent tracer molecule, ... Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2011
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