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Role of Ubiquitin C-Terminal Hydrolase-L1 in Antipolyspermy Defense of Mammalian Oocytes
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SYSNO ASEP 0345745 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Role of Ubiquitin C-Terminal Hydrolase-L1 in Antipolyspermy Defense of Mammalian Oocytes Author(s) Šušor, Andrej (UZFG-Y) RID, ORCID
Lišková, Lucie (UZFG-Y)
Toralová, Tereza (UZFG-Y) ORCID
Pavlok, Antonín (UZFG-Y) RID
Pivoňková, Kateřina (UZFG-Y)
Karabínová, Pavla (UZFG-Y) RID
Lopatářová, M. (CZ)
Šutovský, P. (US)
Kubelka, Michal (UZFG-Y) RID, ORCIDSource Title Biology of Reproduction. - : Oxford University Press - ISSN 0006-3363
Roč. 82, č. 6 (2010), s. 1151-1161Number of pages 11 s. Language eng - English Country US - United States Keywords cortical granule ; deubiquitinating ; fertilization Subject RIV ED - Physiology R&D Projects GA524/07/1087 GA ČR - Czech Science Foundation (CSF) GP524/09/P435 GA ČR - Czech Science Foundation (CSF) GD204/09/H084 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000277964500015 DOI 10.1095/biolreprod.109.081547 Annotation The ubiquitin-proteasome system regulates many cellular processes via proteasomal degradation of ubiquitinated proteins. Ubiquitin C-terminal hydrolase-L1 (UCHL1), an abundant protein in mammalian oocytes, has hydrolytic activity as a monomer. In its dimeric and oligomeric form, UCHL1 acts as an ubiquitin ligase. Recently was found that, deficiency in UCHL1 coincides with polyspermic fertilization; however, the role of UCHL1 in such process remains unclear. After in vitro fertilization, a high rate of polyspermy in bovine zygotes was induced by UCHL1 inhibitors and decreased level of the monomeric as well as polymeric ubiquitin pool was detected. In addition, presumptive UCHL1 oligomers were formed after UCHL1 inhibition with subsequent increase of K63-linked polyubiquitin chains in oocytes. Moreover, both migration of cortical granules (CGs) toward the cortex during oocyte maturation and fertilization-induced extrusion of CGs were impaired after UCHL1 inhibition. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2011
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