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Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic
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SYSNO ASEP 0345538 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Novel spirochetes isolated from mosquitoes and black flies in the Czech Republic Author(s) Šikutová, Silvie (UBO-W) RID, SAI, SAI, ORCID
Halouzka, Jiří (UBO-W) SAI
Mendel, Jan (UBO-W) RID, SAI, SAI, ORCID
Knoz, J. (CZ)
Rudolf, Ivo (UBO-W) RID, ORCID, SAINumber of authors 5 Source Title Journal of Vector Ecology - ISSN 1081-1710
Roč. 35, č. 1 (2010), s. 50-55Number of pages 6 s. Language eng - English Country US - United States Keywords Spirochetes ; mosquitoes ; black flies ; tabanid flies ; Czech Republic Subject RIV FN - Epidemiology, Contagious Diseases ; Clinical Immunology R&D Projects GA206/00/1234 GA ČR - Czech Science Foundation (CSF) GA206/03/0726 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z60930519 - UBO-W (2005-2011) UT WOS 000278574900007 EID SCOPUS 77952033022 DOI 10.1111/j.1948-7134.2010.00057.x Annotation During the years 1999-2002, a total of 4,898 individuals of 26 species of hematophagous insects (4,149 mosquitoes, 583 black flies, and 166 tabanid flies) was examined for the presence of spirochetes using dark-field microscopy. There was an overall recovery of spirochetes from the midguts of Culicidae and Simuliidae of 23.5% and 11.4%, respectively. Spirochetes were not detected in Tabanidae. Seven spirochetal strains have been successfully recovered from mosquitoes and black flies: BR149 (Culex pipiens), BR151 (Cx. pipiens), BR173 (Cx. pipiens), BR177 (Cx. pipiens), BR193 (Aedes cinereus), BR208 (Cx. pipiens), and BR231 (Simulium noelleri). The strains have been adapted to laboratory conditions (BSK-H Complete medium). Their preliminary determination based on 16S rRNA gene sequencing has shown that they differ from the Lyme disease spirochete Borrelia burgdorferi sensu lato as well as other members of the Order Spirochaetales indicating novel bacterial species in the Family Spirochaetaceae. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2011
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