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Second harmonic generation from thick leaves using the two-photon laser scanning microscope
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SYSNO ASEP 0342935 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Second harmonic generation from thick leaves using the two-photon laser scanning microscope Author(s) Reshak, Ali H (UEK-B) Number of authors 1 Source Title Micron. - : Elsevier - ISSN 0968-4328
Roč. 4, č. 40 (2009), s. 455-462Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords Duckweed Lemna minuta Subject RIV BO - Biophysics CEZ AV0Z60870520 - UEK-B (2005-2011) UT WOS 000265777000008 DOI 10.1016/j.micron.2008.12.008 Annotation A laser-scanning microscope using Second harmonic generation (SHG) as a probe is shown to produce high-resolution images of duckweed Lemna minuta leaves. These leaves are multi-cell layer thick. Second harmonic light is generated by a tightly focused short pulse laser beam and is collected by two objectives yielding forward and backward second harmonic digital images. This measurement shows that the signal of the second harmonic imaging in the forward and backward directions depends on the thickness of the chloroplast and that the forward-SH image was brighter than the backward-SH image. The image intensity also depended on the orientation of the chloroplast in relation to the illuminating polarization direction. Their light-induced re-orientation which was affected by the intensity of the illumination could be observed during the experiments. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2011
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