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Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers
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SYSNO ASEP 0342761 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers Author(s) Kulakowska-Zań, Anna (UFCH-W)
Jurkiewicz, Piotr (UFCH-W) RID, ORCID
Sýkora, Jan (UFCH-W) RID
Benda, Aleš (UFCH-W) RID, ORCID
Mely, Y. (FR)
Hof, Martin (UFCH-W) RID, ORCIDSource Title Journal of Fluorescence. - : Springer - ISSN 1053-0509
Roč. 20, č. 2 (2010), s. 563-569Number of pages 7 s. Language eng - English Country US - United States Keywords flip-flop dynamics ; lateral diffusion ; fluorescence lifetime tuning Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GEMEM/09/E006 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000275421600016 DOI 10.1007/s10895-009-0581-9 Annotation In the present work we introduce a straightforward fluorescent assay that can be applied in studies of the transbilayer movement (flip-flop) of fluorescent lipid analogues across supported phospholipid bilayers (SPBs). The assay is based on the distance dependent fluorescence quenching by light absorbing surfaces. Applied to SPBs this effect leads to strong differences in fluorescence lifetimes when the dye moves from the outer lipid leaflet to the leaflet in contact with the support. Herein, we present the basic principles of this novel approach, and comment on its advantages over the commonly used methods for investigating flip-flop dynamics across lipid bilayers. We test the assay on the fluorescent lipid analog Atto633-DOPE and the 3-hydroxyflavone F2N12S probe in SPBs composed of DOPC/ DOPS lipids. Moreover, we compare and discuss the flip-flop rates of the probes with respect to their lateral diffusion coefficients. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2011
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