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Locust adipokinetic hormone mobilizes diacylglycerols selectively
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SYSNO ASEP 0341363 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Locust adipokinetic hormone mobilizes diacylglycerols selectively Author(s) Tomčala, Aleš (UOCHB-X) RID
Bártů, Iva (BC-A)
Šimek, Petr (BC-A) RID, ORCID
Kodrík, Dalibor (BC-A) RID, ORCIDNumber of authors 4 Source Title Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology. - : Elsevier - ISSN 1096-4959
Roč. 156, č. 1 (2010), s. 26-32Number of pages 7 s. Language eng - English Country US - United States Keywords AKH ; lipid ; diacylglycerol Subject RIV ED - Physiology R&D Projects GA522/07/0788 GA ČR - Czech Science Foundation (CSF) GAP501/10/1215 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000276734500005 DOI 10.1016/j.cbpb.2010.01.015 Annotation The diacylglycerols (DG) molecular species and their fatty acid (FA) composition were investigated by electrospray mass spectrometry (ESI-MS) and by gas chromatography with flame ionisation detection (GC-FID) in haemolymph of Locusta migratoria after application of adipokinetic hormones (AKH). The analyses showed (1) a heterogeneous distribution of individual DGs in haemolymph after the AKH application. The results revealed that mobilization of the DGs is specific and that palmitic (C16:0) and oleic acid (C18:1) residues form about 20% of the total mobilized DGs. (2) Analysis of fat body TGs revealed that all Locmi-AKHs mobilize the DGs selectively with the preference of those possessing the C18 and C16 FAs. (3) A distribution of FAs in the DG structures indicated a certain degree of Locmi-AKH selectivity toward the mobilized DGs and hence the FAs. The Locmi-AKH-I significantly prefers mobilization unsaturated, while Locmi-AKH-II and -III mobilization of saturated FAs. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2011
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