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Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries
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SYSNO ASEP 0337706 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries Title Tvorba zdrojů pro genomiku homeologních chromozómů skupiny 3 pšenice: BAC knihovny specifické pro chromozóm 3AS a 3DS Author(s) Šafář, Jan (UEB-Q) RID, ORCID
Šimková, Hana (UEB-Q) RID, ORCID
Kubaláková, Marie (UEB-Q) RID
Suchánková, Pavla (UEB-Q) RID
Čihalíková, Jarmila (UEB-Q) RID
Bartoš, Jan (UEB-Q) RID, ORCID
Fiocchetti, F. (IT)
Roselli, M. (IT)
Gill, B. S. (US)
Doležel, Jaroslav (UEB-Q) RID, ORCID
Lucretti, S. (IT)Source Title Journal of Genetics & Breeding - ISSN 0394-9257
Roč. 61, 1-2 (2009), s. 151-160Number of pages 10 s. Language eng - English Country IT - Italy Keywords BAC library ; Flow sorting ; Homoeologous chromosomes Subject RIV GE - Plant Breeding R&D Projects GD521/05/H013 GA ČR - Czech Science Foundation (CSF) GA521/06/1723 GA ČR - Czech Science Foundation (CSF) GA521/07/1573 GA ČR - Czech Science Foundation (CSF) LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC08025 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation In bread wheat comprehensive genome analysis is complicated by its very large genome (~17 Gbp), polyploid nature and high repetitive sequence content. Using flow cytometry allows dissecting the wheat genome to individual chromosomes. Flow sorted chromosomes were used already to construct several grain BAC libraries including those from chromosome 3B and chromosome arms 1BS and 1RS. In the present work we compare the construction of the 3AS/3DS BAC libraries in respect to our new improved protocol which made two-sized selection feasible. A minimal tiling path of chromosome 3B has already been established. Construction of the 3AS- and 3DS-specific BAC libraries represent significant step toward completing BAC resources for the homoeologous chromosome group 3 of wheat and accelerate the development of sequence-ready physical contig maps and gene cloning, as well as comparative analyses aiming at revealing the genome changes accompanying the evolution of homoeologous chromosome group 3. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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