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AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis
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SYSNO ASEP 0337486 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis Author(s) Zhang, J. (CN)
Vaňková, Radomíra (UEB-Q) RID, ORCID
Malbeck, Jiří (UEB-Q) RID, ORCID
Dobrev, Petre (UEB-Q) RID, ORCID
Xu, Y. (CN)
Chong, K. (CN)
Neff, M. M. (US)Source Title PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 4, č. 12 (2009), Art_no_-e8236Number of pages 11 s. Language eng - English Country US - United States Keywords TRANSGENIC TOBACCO PLANTS ; OXIDASE/DEHYDROGENASE GENES ; ISOPENTENYL TRANSFERASE Subject RIV ED - Physiology R&D Projects GA206/06/1306 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000272829800020 DOI 10.1371/journal.pone.0008236 Annotation Two SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUS indicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(∆2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2010
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