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Comparison of Metallothionein Detection by Using Brdicka Reaction and Enzyme-Linked Immunosorbent Assay Employing Chicken Yolk Antibodies
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SYSNO ASEP 0335259 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparison of Metallothionein Detection by Using Brdicka Reaction and Enzyme-Linked Immunosorbent Assay Employing Chicken Yolk Antibodies Author(s) Křížková, S. (CZ)
Bláhová, P. (CZ)
Nakielna, J. (CZ)
Fabrik, I. (CZ)
Adam, V. (CZ)
Eckschlager, T. (CZ)
Beklová, M. (CZ)
Svobodová, Z. (CZ)
Horák, Vratislav (UZFG-Y) RID, ORCID
Krížek, R. (CZ)Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 21, č. 23 (2009), s. 2575-2583Number of pages 9 s. Language eng - English Country DE - Germany Keywords Metallothionein ; Differential pulse voltammetry ; ELISA Subject RIV CG - Electrochemistry CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000272738800010 DOI 10.1002/elan.200900243 Annotation In this study, two methods for metallothioneins determination in a biological sample were compared. Human and pig blood serum samples and liver and kidney samples from carps (Cyprinus carpio) were analyzed by enzyme-linked immunosorbent assay and differential pulse voltammetry Brdicka´s reaction. The results obtained by these two methods were in good agreement. The correlation coefficients were 0.90 for fish liver and kidney samples, 0.91 for pig blood serum and 0.93 for human blood serum. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2010
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