Number of the records: 1
Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase
- 1.
SYSNO ASEP 0334909 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Kinetoplastid guide RNA biogenesis is dependent on subunits of the mitochondrial RNA binding complex 1 and mitochondrial RNA polymerase Title Biogeneze kinetoplastidové guide RNA závisí na podjednotkách mitochondriálního RNA vazebného komplexu 1 a na mitochondriální RNA polymeráze Author(s) Hashimi, Hassan (BC-A) RID, ORCID
Číčová, Zdeňka (BC-A)
Novotná, Lucie (BC-A)
Wen, Y.-Z. (CN)
Lukeš, Julius (BC-A) RID, ORCIDSource Title RNA. - : Cold Spring Harbor Laboratory Press - ISSN 1355-8382
Roč. 15, č. 4 (2009), s. 588-599Number of pages 11 s. Language eng - English Country US - United States Keywords RNA editing ; guide RNA ; mitochondrion ; trypanosome Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/09/1667 GA ČR - Czech Science Foundation (CSF) IAA500960705 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC07032 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 2B06129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GD524/03/H133 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000264280100008 Annotation The mitochondrial RNA binding complex 1 (MRB1) is a recently discovered set of proteins associated with the TbRGG1 in Trypanosoma brucei. Based on the phenotype caused by downregulation of this protein, it was proposed to play an unspecified RNA editing role. RNAi-silencing of three newly characterized protein subunits, guide RNA associated proteins (GAPs) 1 and 2 plus a predicted RNA helicase, show they are essential for cell growth in the insect stage. Furthermore, their downregulation leads to inhibition of editing in only those mRNAs for which minicircle-encoded guide (g) RNAs are required. All three proteins are necessary for the expression of the minicircle-encoded gRNAs. Moreover, down-regulation of a fourth assayed putative MRB1 subunit, Nudix hydrolase, does not appear to destabilize gRNAs but has a general impact on the stability of maxicircle-encoded RNAs. We also demonstrate that the same mt RNA polymerase may also have a role in expression of the minicircle-encoded gRNAs. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
Number of the records: 1