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Optical Saturation as a Versatile Tool to Enhance Resolution in Confocal Microscopy
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SYSNO ASEP 0333796 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Optical Saturation as a Versatile Tool to Enhance Resolution in Confocal Microscopy Title Optická saturace jako nástroj ke zvýšení rozlišení konfokaního mikroskopu Author(s) Humpolíčková, Jana (UFCH-W) RID
Benda, Aleš (UFCH-W) RID, ORCID
Enderlein, J. (DE)Source Title Biophysical Journal. - : Cell Press - ISSN 0006-3495
Roč. 97, č. 9 (2009), s. 2623-2629Number of pages 7 s. Language eng - English Country US - United States Keywords fluorescence microscopy ; reconstruction microscopy ; cassettes Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects KJB400400904 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000271454000029 DOI 10.1016/j.bpj.2009.08.002 Annotation One of the most actively developing areas in fluorescence microscopy is the achievement of spatial resolution below Abbe's diffraction limit, which restricts the resolution to several hundreds of nanometers. Most of the approaches in use at this time require a complex optical setup, a difficult mathematical treatment, or usage of dyes with special photophysical properties. In this work, we present a new, to our knowledge, approach in confocal microscopy that enhances the resolution moderately but is both technically and computationally simple. As it is based on the saturation of the transition from the ground state to the first excited state, it is universally applicable with respect to the dye used. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2010
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