Number of the records: 1
Changes in Electrostatic Surface Potential of Na+/K+-ATPase Cytoplasmic Headpiece Induced by Cytoplasmic Ligand(s) Binding
- 1.
SYSNO ASEP 0333696 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Changes in Electrostatic Surface Potential of Na+/K+-ATPase Cytoplasmic Headpiece Induced by Cytoplasmic Ligand(s) Binding Title Změny v povrchovém elektrostatickém potenciálu velké cytoplazmatické smyčky Na+/K+ ATPázy vyvolané vazbou cytoplazmatických ligandů Author(s) Kubala, M. (CZ)
Gryčová, Lenka (FGU-C) RID
Lánský, Zdeněk (FGU-C)
Sklenovský, P. (CZ)
Janovská, M. (CZ)
Otyepka, M. (CZ)
Teisinger, Jan (FGU-C) RIDSource Title Biophysical Journal. - : Cell Press - ISSN 0006-3495
Roč. 97, č. 6 (2009), s. 1756-1764Number of pages 9 s. Language eng - English Country US - United States Keywords Na(+)/K(+)-ATPase ; intrinsic fluorescence ; quenching Subject RIV BO - Biophysics R&D Projects GA303/07/0915 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000270380800027 DOI 10.1016/j.bpj.2009.07.002 Annotation A set of single-tryptophan mutants of the Na(+)/K(+)-ATPase isolated, large cytoplasmic loop connecting transmembrane helices M4 and M5 (C45) was prepared to monitor effects of the natural cytoplasmic ligands (i.e., Mg(2+) and/or ATP) binding. We introduced a novel method for the monitoring of the changes in the electrostatic surface potential (ESP) induced by ligand binding, using the quenching of the intrinsic tryptophan fluorescence by acrylamide or iodide Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2010
Number of the records: 1