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GAR22: A novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells
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SYSNO ASEP 0333660 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title GAR22: A novel target gene of thyroid hormone receptor causes growth inhibition in human erythroid cells Author(s) Gamper, I. (DE)
Koh, K.-R. (DE)
Ruau, D. (DE)
Ullrich, K. (DE)
Bartůňková, Jana (UMG-J)
Piroth, D. (DE)
Hacker, C. (DE)
Bartůněk, Petr (UMG-J) RID
Zenke, M. (DE)Number of authors 9 Source Title Experimental Hematology. - : Elsevier - ISSN 0301-472X
Roč. 37, č. 5 (2009), s. 539-548Number of pages 10 s. Language eng - English Country US - United States Keywords Thyroid hormone receptor ; GAR22 ; erythropoiesis Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC06077 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000265626900002 DOI 10.1016/j.exphem.2009.02.002 Annotation Thyroid hormone receptors (TRs) are ligand-dependent transcription factors with a major impact on erythroid cell development. Here we investigated TR activity on red cell gene expression using a genome-wide approach with DNA microarrays and identified TR target genes. Ligand-activated TR effectively accelerated red cell progenitor differentiation in vitro concomitantly with inducing growth arrest. We demonstrate that activated TR induced specific gene expression patterns of up- or down-regulated genes, including distinct clusters associated with accelerated differentiation in response to treatment. Mining for T3 induced genes identified BTEB1 and GAR22 as TR target genes. BTEB1 is a known TR target gene while GAR22, initially identified as a putative tumor suppressor, represents a novel TR target gene. We demonstrate that ectopic GAR22 expression in red cell progenitors lengthens the cell cycle and causes growth inhibition, but leaves red cell gene expression unaffected. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
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