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Mapping of HIV-1 integrase preferences for target site selection with various oligonucleotides
- 1.0333523 - ÚOCHB 2010 RIV US eng J - Journal Article
Snášel, Jan - Rosenberg, Ivan - Pačes, Ondřej - Pichová, Iva
Mapping of HIV-1 integrase preferences for target site selection with various oligonucleotides.
Archives of Biochemistry and Biophysics. Roč. 488, č. 2 (2009), s. 153-162. ISSN 0003-9861. E-ISSN 1096-0384
R&D Projects: GA MŠMT 1M0508; GA MŠMT(CZ) LC06077
Institutional research plan: CEZ:AV0Z40550506
Keywords : HIV integrase * strand transfer reaction * oligonucleotide substrates
Subject RIV: CE - Biochemistry
Impact factor: 3.046, year: 2009
HIV integrase (IN) catalyzes the insertion of proviral DNA into the host cell chromosome. The integration site preference has been shown to be very diverse. We mapped the HIV integrase strand transfer reaction requirements using various short oligonucleotides (ON) that mimic the target DNA. We tested double stranded DNA dodecamers and hairpin-loop-forming oligonucleotides of different length. The integration biases with all target DNA structures tested were significantly influenced by changes in the resulting secondary ON structures.
Permanent Link: http://hdl.handle.net/11104/0178483
Number of the records: 1