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Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio
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SYSNO ASEP 0331350 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern hybridizatio Title Analýza telomer u ploštěnců a vrtejšů pomocí FISH a Southernovy hybridizace Author(s) Bombarová, M. (SK)
Vítková, Magda (BC-A) RID
Špakulová, M. (SK)
Koubková, B. (CZ)Number of authors 4 Source Title Genome. - : Canadian Science Publishing - ISSN 0831-2796
Roč. 52, č. 11 (2009), s. 897-903Number of pages 7 s. Language eng - English Country CA - Canada Keywords Acanthocephala ; Platyhelminthes ; chromosomes Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA206/06/1860 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50070508 - ENTU-I, BC-A (2005-2011) UT WOS 000272206700001 DOI 10.1139/G09-063 Annotation We examined the composition of telomeres of parasitic worms, representatives of flatworm groups Monogenea and Cestoda Platyhelminthes), and thorny-headed worms (Syndermata: Acanthocephala), by fluorescence in situ hybridization (FISH) with different telomeric repeat probes. Our results show that the (TTAGGG)n sequence, supposed to be the ancestral telomere repeat motif of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda (Caryophyllaeus laticeps, Caryophyllaeides fennica, Nippotaenia mogurndae) but not in Acanthocephala Pomphorhynchus laevis and P. tereticollis). In the Pomphorhynchus species, no hybridisation signals were obtained either with the ‘nematode’ (TTAGGC)n, ‘arthropod’ (TTAGG)n and bdelloid (TGTGGG)n, telomeric probes using the FISH with their chromosomes and Southern hybridization with P. laevis DNA. Therefore, we suggest that parasitic Acanthocephala have evolved yet unknown telomeric repeat motif or different mechanism of telomere maintenance. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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