Phytoplasma associated with witches´-broom disease of Ulmus minor in the Czech Republic. Electron microscopy and molecular characterization

0329691 - BC 2010 RIV CZ eng J - Journal Article
Navrátil, M. - Šafářová, D. - Válová, P. - Fránová, Jana - Šimková, Marie
Phytoplasma associated with witches´-broom disease of Ulmus minor in the Czech Republic. Electron microscopy and molecular characterization.
[Fytoplasma asociovaná s příznaky čarovějníků Ulmus minor v České republice. Elektronová mikroskopie a molekulární charakterizace.]
Folia Microbiologica. Roč. 54, č. 1 (2009), s. 37-42. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA AV ČR(CZ) 1QS500510558
Institutional research plan: CEZ:AV0Z50510513
Keywords : Ulmus minor * Candidatus Phytoplasma ulmi * PCR * sequencing
Subject RIV: EE - Microbiology, Virology
Impact factor: 0.978, year: 2009

Witches´-broom disease of Ulmus minor was observed in south Moravia in 1997-2007. The presence of phytoplasma-like bodies was observed by TEM of phloem tissue. Sequence analyses of the 16S–23S ribosomal operon (1852 bp) allowed for the classification of the detected phytoplasmas in the elm yellows group, but its position remained on the boundary of the 16SrV-A and 16SrV-C ribosomal subgroups. Sequence analyses of the ribosomal protein of the rpl22-rps3 and secY genes lead to further classification and revealed the phytoplasmas’ affiliations to the ‘Candidatus Phytoplasma ulmi’.

Příznaky čarovějníku byly pozorovány na jilmech na jižní Moravě v průběhu vizuálních inspekcí v letech 1997 – 2007. Na ultratenkých řezech cévních svazků byly nalezeny částice fytoplazem. Přítomnost fytoplazem byla potvrzena molekulárními metodami. Fytoplazmy byly klasifikovány jako ‘Candidatus Phytoplasma ulmi’.
Permanent Link: http://hdl.handle.net/11104/0175657