Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration

0329247 - FGÚ 2010 RIV IT eng C - Conference Paper (international conference)
Čapek, Martin - Brůža, Petr - Kocandová, Lucie - Janáček, Jiří - Kubínová, Lucie - Hozman, J. - Kneppo, P. - Vagnerová, R.
Volume Reconstruction Using Confocal Microscopy Images and Correction of Deformations by Elastic Registration.
[Objemová rekonstrukce s využitím konfokálních obrazů a korekce deformací elastickou registrací.]
Stereology and Image Analysis. Bologna: Esculapio, 2009 - (Capasso, V.; Aletti, G.; Micheletti, A.), s. 538-543. ISBN 978-88-7488-310-3.
[European Congress of Stereology and Image Analysis /10./. Milano (IT), 22.06.2009-26.06.2009]
R&D Projects: GA AV ČR(CZ) IAA100110502; GA ČR(CZ) GA102/08/0691; GA ČR GA304/09/0733; GA MŠMT(CZ) LC06063
Institutional research plan: CEZ:AV0Z50110509
Keywords : 3D reconstruction * elastic registration * 3D visualization * confocal microscopy
Subject RIV: JC - Computer Hardware ; Software

A set of methods leading to volume reconstruction of biological specimens larger than the field of view of a confocal laser scanning microscope (CLSM) is presented. Large tissue specimens are cut into thin physical slices and volume data sets are captured from all studied physical slices by CLSM. Overlapping spatial tiles of the same physical slice are stitched in horizontal direction. Image volumes of successive physical slices are linked in axial direction by applying an elastic registration algorithm to compensate for deformations due to cutting the specimen. We present a method enabling us to keep true object morphology using a-priori information about the shape and size of the specimen, available from images of the cutting planes captured by a USB light microscope immediately before cutting the specimen by a microtome. The errors introduced by elastic registration are evaluated using a stereological point counting method and the Procrustes distance

Popisujeme soubor metod vedoucí k objemové rekonstrukci z konfokálních dat nasnímaných z velkých biologických preparátů. Objemovou rekonstrukci využíváme pro vizualizaci biologických preparátů, které jsou větší, než je velikost zorného pole a/nebo silnější v tloušťce než je hloubka průniku laserového paprsku konfokálního mikroskopu. Metody demonstrujeme rekonstrukcí střední části 17-denního embrya laboratorní krysy
Permanent Link: http://hdl.handle.net/11104/0175335