Number of the records: 1
Magnesium interference and different efficiencies of diastereoisomeric cluster formation in phenylalanine enantiomeric discrimination by the kinetic method
- 1.
SYSNO ASEP 0328370 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Magnesium interference and different efficiencies of diastereoisomeric cluster formation in phenylalanine enantiomeric discrimination by the kinetic method Title Interference hořčíku při stanovení diastereomerní čistoty fenylalaninu , kinetickou metodou Author(s) Ranc, V. (CZ)
Havlíček, Vladimír (MBU-M) RID, ORCID
Bednář, P. (CZ)
Lemr, Karel (MBU-M) RID, ORCIDSource Title International Journal of Mass Spectrometry. - : Elsevier - ISSN 1387-3806
Roč. 280, 1-3 (2009), s. 213-217Number of pages 5 s. Language eng - English Country NL - Netherlands Keywords enantiomeric dicrimination ; kinetic method ; mass spectrometry Subject RIV CB - Analytical Chemistry, Separation R&D Projects LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA203/07/0765 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000263195500034 DOI 10.1016/j.ijms.2008.09.009 Annotation The kinetic method has been applied for determination of d-Phe/l-Phe enantiomeric ratio. Discrimination of enantiomers was inferred from product ion mass spectra of trimeric cluster ions containing the analyte (l,d-Phe), Cu2 as a central metal and l-Trp as a chiral reference ligand. Unsatisfactory quantitative results achieved on an ion trapwere rationalized by high-resolution mass spectrometry. The formation of Mg2-containing cluster isobaric to trimeric cluster [Cu(l-Trp)2Phe] was observed. Interference like this was identified as a possible reason for deterioration of quantitative low-resolution mass spectrometric analyses of real-world samples based on the kinetic method. Cation-exchangerwas used for easy removal of magnesium from a sample and improvement of quantitation. Chiral dependence of formation of the Cu2 containing trimeric clusterwas also observed. Heterochiral diastereoisomeric ions were created less effectively Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
Number of the records: 1