Gene expression during minor genome activation in preimplantation bovine development

0328317 - ÚŽFG 2010 RIV US eng J - Journal Article
Kaňka, Jiří - Kepková, Kateřina - Němcová, Lucie
Gene expression during minor genome activation in preimplantation bovine development.
Theriogenelogy. Roč. 72, - (2009), s. 572-583. ISSN 0093-691X. E-ISSN 1879-3231
R&D Projects: GA ČR GA523/06/1226
Institutional research plan: CEZ:AV0Z50450515
Keywords : developmental biology * embryo * gene expression * real-time RT-PCR
Subject RIV: GI - Animal Husbandry ; Breeding
Impact factor: 2.073, year: 2009

The main goal of this study was to identify mRNA transcripts, whose content increases during bovine minor embryonic genome activation. We compared the gene expression profile of the bovine 4-cell stage embryo and MII oocyte using the technique of suppression subtractive hybridization. Differentially expressed amplicons were subcloned and 60 of them were sequenced. The resulting DNA sequences were compared with GenBank databases using Blast search. The expression of five differentially expressed genes with an apparent function in cell cycle progression, chromatin remodeling, and splicing or translation initiation was further characterized by a real-time RT-PCR. Splicing factor, arginine/serine-rich 3 (SRFS3) represents the first described gene with an important function in pre-implantation development, which is also expressed during bovine minor genome activation and it is alpha-amanitin sensitive during this period. All described genes can play an important role in the pre-implantation development of bovine embryos.
Permanent Link: http://hdl.handle.net/11104/0174658