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Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases
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SYSNO ASEP 0328051 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases Author(s) Syslová, K. (CZ)
Kačer, P. (CZ)
Kuzma, Marek (MBU-M) ORCID, RID
Najmanová, V. (CZ)
Fenclová, Z. (CZ)
Vlčková, Š. (CZ)
Lebedová, J. (CZ)
Pelclová, D. (CZ)Source Title Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 877, č. 24 (2009), s. 2477-2486Number of pages 10 s. Language eng - English Country NL - Netherlands Keywords malondialdehyde ; 4-Hydroxynonenal ; exhaled breath condensate Subject RIV EE - Microbiology, Virology R&D Projects NR9338 GA MZd - Ministry of Health (MZ) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000269023200010 DOI 10.1016/j.jchromb.2009.06.008 Annotation Sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F2a, malondialdehyde and 4-hydroxynonenal. The method consisted of a pre-treatment part a solid-phase extraction, for rapid and effective isolation of biomarkers from body fluids (exhaled breath condensate, plasma and urine) and the detection method LC–ESI-MS/MS, where the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method was characterized by the following parameters: the imprecision was below 14.3 percent, the mean inaccuracy was determined to be lower than 13.1 percent Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
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