Immobility of phycobilins in the thylakoid lumen of a cryptophyte suggests that protein diffussion in the lumen is very restricted

0327455 - MBÚ 2010 RIV GB eng J - Journal Article
Kaňa, Radek - Prášil, Ondřej - Mullineaux, C.W.
Immobility of phycobilins in the thylakoid lumen of a cryptophyte suggests that protein diffussion in the lumen is very restricted.
FEBS Letters. Roč. 583, č. 4 (2009), s. 670-674. ISSN 0014-5793. E-ISSN 1873-3468
R&D Projects: GA AV ČR IAA608170603; GA ČR GP206/09/P094
Institutional research plan: CEZ:AV0Z50200510
Keywords : cryptophyte (Rhodomonas salina) * fluorescence recovery after photobleaching * protein diffusion
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.541, year: 2009

The thylakoid lumen is an important photosynthetic compartment which is the site of key steps in photosynthetic electron transport. The fluidity of the lumen could be a major constraint on photosynthetic electron transport rates. We used Fluorescence Recovery After Photobleaching in cells of the cryptophyte alga Rhodomonas salina to probe the diffusion of phycoerythrin in the lumen and chlorophyll complexes in the thylakoid membrane. In neither case was there any detectable diffusion over a timescale of several minutes. This indicates very restricted phycoerythrin mobility. This may be a general feature of protein diffusion in the thylakoid lumen
Permanent Link: http://hdl.handle.net/11104/0174268