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Revesible coupling of individual phycobiliprotein isoforms during state transitions in the cyanobacterium .i.Trichodesmium./i. analysed by single-cell fluorescence kinetic measurements
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SYSNO ASEP 0327238 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Revesible coupling of individual phycobiliprotein isoforms during state transitions in the cyanobacterium Trichodesmium analysed by single-cell fluorescence kinetic measurements Title Reverzní spojení izomorfů fykobiliproteinů u sinice Trichodesmium měřené stanovením kinetiky fluorescence buněk Author(s) Küpper, H. (DE)
Andresen, E. (DE)
Wiegert, S. (DE)
Šimek, Miloslav (BC-A) RID, ORCID
Leitenmaier, B. (DE)
Šetlík, Ivan (MBU-M)Source Title Biochimica Et Biophysica Acta-Bioenergetics. - : Elsevier - ISSN 0005-2728
Roč. 1787, č. 3 (2009), s. 155-167Number of pages 13 s. Language eng - English Country NL - Netherlands Keywords fluorescence kinetic microscopy ; in vivo spectroscopy ; nitrogen fixation Subject RIV EH - Ecology, Behaviour CEZ AV0Z60660521 - UPB-H, BC-A (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000263992800003 DOI 10.1016/j.bbabio.2009.01.001 Annotation In the non-heterocyst, marine cyanobacterium Trichodesmium nitrogen fixation is confined to the photoperiod and occurs coevally with oxygenic photosynthesis although nitrogenase is irreversibly inactivated by oxygen. In previous studies it was found that regulation of photosynthesis for nitrogen fixation involves Mehler reaction and various activity states with reversible coupling of photosynthetic components. We now investigated these activity states in more detail. Spectrally resolved fluorescence kinetic measurements of single cells revealed that they were related to alternate uncoupling and coupling of phycobilisomes from and to the photosystems, changing the effective cross-section of PSII. Therefore, we isolated and purified the phycobiliproteins of Trichodesmius via ion exchange chromatography and recorded their UV/VIS absorption, fluorescence excitation and fluorescence emission spectra. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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