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Biomarkers of Aspergillus spores: Strain typing and protein identification
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SYSNO ASEP 0327084 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biomarkers of Aspergillus spores: Strain typing and protein identification Title Biomarkery spór kmene Aspergillus: určování kmenů a identifikace proteinů Author(s) Šulc, Miroslav (MBU-M) RID, ORCID
Pešlová, Kateřina (MBU-M)
Žabka, Martin (MBU-M)
Hajdúch, M. (CZ)
Havlíček, Vladimír (MBU-M) RID, ORCIDSource Title International Journal of Mass Spectrometry. - : Elsevier - ISSN 1387-3806
Roč. 280, 1-3 (2009), s. 162-168Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords aspergillus ; spore ; protein Subject RIV EE - Microbiology, Virology R&D Projects LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GP203/05/P575 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000263195500025 DOI 10.1016/j.ijms.2008.08.012 Annotation The direct analysis of intact fungal spores of twenty four Aspergillus species using MALDI-TOF mass spectrometry provided a reproducible and fingerprint spectra demonstrating the capability of the MALDI-TOF approach to type and characterize different fungal strains within the Aspergillus genus. Mass spectra of intact fungal spores provided signals mostly below 20 kDa. The minimum material amount represented 0.3 microg (10,000 spores). Proteins with higher molecular weight were detected by 1D-PAGE. Eleven proteins were identified from three selected strains in the range 5–25 kDa by the proteomic approach. Hemolysin and hydrophobin have the highest relevance in host–pathogen interactions Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2010
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