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The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering
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SYSNO ASEP 0326485 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The cultivation of human multipotent mesenchymal stromal cells in clinical grade medium for bone tissue engineering Title Kultivace lidských multipotentních mesenchymálních buněk kostní dřeně pro tkáňové inženýrství kosti v médiu pro klinické použití Author(s) Pytlík, R. (CZ)
Stehlík, D. (CZ)
Soukup, T. (CZ)
Kalbáčová, M. (CZ)
Rypáček, František (UMCH-V) RID
Trč, T. (CZ)
Mulinková, Katarína (UMCH-V)
Michnová, P. (CZ)
Kideryová, L. (CZ)
Živný, J. (CZ)
Klener, P.Jr. (CZ)
Veselá, R. (CZ)
Trněný, M. (CZ)
Klener, P. (CZ)Source Title Biomaterials. - : Elsevier - ISSN 0142-9612
Roč. 30, č. 20 (2009), s. 3415-3427Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords tissue engineering ; multipotent mesenchymal stromal cells ; human serum Subject RIV FD - Oncology ; Hematology R&D Projects ND7448 GA MZd - Ministry of Health (MZ) CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000266744100007 DOI 10.1016/j.biomaterials.2009.03.001 Annotation Clinical application of human multipotent mesenchymal stromal cells (hMSCs) requires their expansion to be safe and rapid. We aimed to develop an expansion protocol which would avoid xenogeneic proteins, including fetal calf serum (FCS), and which would shorten the cultivation time and avoid multiple passaging. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2010
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