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Analysis of insect triacylglycerols using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry
- 1.0326454 - ÚOCHB 2010 RIV DE eng J - Journal Article
Kofroňová, Edita - Cvačka, Josef - Jiroš, Pavel - Sýkora, D. - Valterová, Irena
Analysis of insect triacylglycerols using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry.
European Journal of Lipid Science and Technology. Roč. 111, č. 5 (2009), s. 519-525. ISSN 1438-7697. E-ISSN 1438-9312
R&D Projects: GA AV ČR IAA4055403; GA MŠMT 2B06007
Institutional research plan: CEZ:AV0Z40550506
Keywords : atmospheric pressure chemical ionization * bumblebees * fat body * NARP-HPLC
Subject RIV: CC - Organic Chemistry
Impact factor: 1.831, year: 2009
An HPLC non-aqueous reversed-phase separation system was adapted for analyzing insect triacylglycerols (TAG). The method uses two conventional Nova-Pak C18 columns connected in series, for a total length of 45 cm. The mobile phase gradient is mixed from acetonitrile and 2-propanol, and the flow rate is programmed from 1.0 to 0.7 mL/min. TAG are detected by atmospheric pressure chemical ionization mass spectrometry. The method ensures efficient separation of isomers and analysis of high-molecular weight TAG with equivalent chain lengths up to 72. The method performance is demonstrated on analysis of TAG isolated from the fat body of the bumblebee Bombus lucorum.
Permanent Link: http://hdl.handle.net/11104/0173556
Number of the records: 1