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Effect of reduced glutathione, surface active agents, and ionic strength on the detection of metallothioneins by using of Brdicka reaction
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SYSNO ASEP 0325004 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of reduced glutathione, surface active agents, and ionic strength on the detection of metallothioneins by using of Brdicka reaction Title Účinek redukovaného glutathionu, povrchově aktivních látek a iontové síly na detekci metalothioneinů stanovených Brdičkovou reakcí Author(s) Křížková, S. (CZ)
Fabrik, I. (CZ)
Adam, V. (CZ)
Kukačka, J. (CZ)
Průša, R. (CZ)
Trnková, L. (CZ)
Strnádel, Ján (UZFG-Y) RID
Horák, Vratislav (UZFG-Y) RID, ORCID
Kizek, R. (CZ)Source Title Electroanalysis. - : Wiley - ISSN 1040-0397
Roč. 21, 3-5 (2009), s. 640-644Number of pages 5 s. Action International Conference on Electroanalysis /12./ Event date 16.06.2008-19.06.2008 VEvent location Prague Country CZ - Czech Republic Event type WRD Language eng - English Country DE - Germany Keywords differential pulse voltammetry ; adsorptive transfer stripping technique ; proteins Subject RIV CG - Electrochemistry CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000264063500057 DOI 10.1002/elan.200804406 Annotation We investigated the effects of two surface active agents (sodium dodecyl sulfate, SDS, and polyoxyethylene sorbitol, Tween-20), ionic strength and reduced glutathione (GSH) on metallothionein (MT) signals measured by adsorptive transfer stripping differential pulse voltammetry Brdicka reaction. The MT signal was more than two hundred times higher than the GSH one at the accumulation time of 120s. The influence of Tween-20 was investigated in a broad concentration range (from 10-8 to 10-11 %, v/v). We found that the catalytic Cat2 signal decreased for more than 60% with increasing Tween-20 content. In the case of using SDS (10-3 – 10-6 M), the increase of Cat2 peak in the presence of the highest SDS concentration was determined. The height of Cat2 peak enhanced with increasing ionic strength mimicking by NaCl concentration. Based on the enhancement of the Cat2 peak we determined the ionic strength of fibroblast samples and recalculated the concentration of MT determined there. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2009
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