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Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications
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SYSNO ASEP 0324504 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Base-modified DNA labeled by [Ru(bpy)3]2+ and [Os(bpy)3]2+ complexes: construction by polymerase incorporation of modified nucleoside triphosphates, electrochemical and luminescent properties, and applications Title DNA obsahující modifikované báze označené [Ru (bpy)3]2+ a [Os(bpy)3]2+ komplexy: konstrukce inkorporacemi modifikovaných nukleosid trofosfátů polymerasou, elektrochemické a luminiscenční vlastnosti a aplikace Author(s) Vrábel, Milan (UOCHB-X) RID, ORCID
Horáková Brázdilová, Petra (BFU-R)
Pivoňková, Hana (BFU-R) ORCID
Kalachová, Lubica (UOCHB-X)
Černocká, Hana (BFU-R) RID, ORCID
Cahová, Hana (UOCHB-X) RID, ORCID
Pohl, Radek (UOCHB-X) RID, ORCID
Šebest, Peter (BFU-R) ORCID
Havran, Luděk (BFU-R) RID, ORCID
Hocek, Michal (UOCHB-X) RID, ORCID
Fojta, Miroslav (BFU-R) RID, ORCIDNumber of authors 11 Source Title Chemistry - A European Journal. - : Wiley - ISSN 0947-6539
Roč. 15, č. 5 (2009), s. 1144-1154Number of pages 11 s. Language eng - English Country DE - Germany Keywords nucleotides ; polymerase ; DNA ; metal complexes Subject RIV CC - Organic Chemistry R&D Projects LC512 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC06035 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA203/07/1195 GA ČR - Czech Science Foundation (CSF) IAA400040901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z40550506 - UOCHB-X (2005-2011) AV0Z50040507 - BFU-R (2005-2011) UT WOS 000263093700012 DOI 10.1002/chem.200801538 Annotation Modified nucleoside triphosphates bearing [Ru (bpy)3]2+ and [Os(bpy)3]2+ complexes attached via acetylene linker were prepared by cross-coupling reactions and were further used for incorporation to DNA by polymerase. The metal complexes were used as luminescent or redox label for detection of DNA. Multi-color redox labeling of DNA by four different labels was also developed. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2009
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