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The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus
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SYSNO ASEP 0323923 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The impact of altered polyprotein ratios on the assembly and infectivity of Mason-Pfizer monkey virus Title Studium vlivu změněných poměrů polyproteinových prekursorů na skládání a infektivitu Mason-Pfizerova opičího viru Author(s) Kohoutová, Z. (CZ)
Rumlová, Michaela (UOCHB-X) RID
Andreánský, M. (US)
Sakalian, M. (US)
Hunter, E. (US)
Pichová, Iva (UOCHB-X) RID, ORCID
Ruml, T. (CZ)Number of authors 7 Source Title Virology. - : Elsevier - ISSN 0042-6822
Roč. 384, č. 1 (2009), s. 59-68Number of pages 10 s. Language eng - English Country US - United States Keywords retrovirus ; assembly ; frameshift ; infectivity Subject RIV CE - Biochemistry R&D Projects 1M0508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) KAN200100801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GESCO/06/E001 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000263039200010 DOI 10.1016/j.virol.2008.10.048 Annotation Most retroviruses employ a frameshift mechanism during polyprotein synthesis to balance appropriate ratios of structural proteins and enzymes. To investigate the requirements for individual precursors in retrovirus assembly, we modified the polyprotein repertoire of Mason-Pfizer monkey virus (M-PMV) to imitate the polyprotein organization of Rous sarcoma virus or Human immunodeficiency virus. For the "Rous-like" virus, assembly was impaired with no incorporation of Gag-Pro-Pol into particles and for the "HIV-like" virus an altered morphogenesis was observed. All the mutants were non-infectious except the "HIV-like", which retained fractional infectivity. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Year of Publishing 2009
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