Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica

Kráľová-Hromadová, I.; Špakulová, M.; Horáčková, Eva; Turčeková, Ĺ.; Novobilský, A.; Beck, R.; Koudela, Břetislav; Marinculić, A.; Rajský, D.; Pybus, M.

Number of the records: 1

Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica

1.

SYSNO ASEP	0321089
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae): intraspecific variation and differentiation from Fasciola hepatica
Title	Sekvenční analýza ribozomálních a mitochondriálních genů motolice velké Fascioloides magna (Trematoda: Fasciolidae): vnitrodruhová variabilita a odlišení od Fasciola hepatica
Author(s)	Kráľová-Hromadová, I. (SK) Špakulová, M. (SK) Horáčková, Eva (BC-A) Turčeková, Ĺ. (SK) Novobilský, A. (CZ) Beck, R. (HR) Koudela, Břetislav (BC-A)_ORCID Marinculić, A. (HR) Rajský, D. (SK) Pybus, M. (CA)
Number of authors	10
Source Title	Journal of Parasitology. - : Allen Press - ISSN 0022-3395 Roč. 94, č. 1 (2008), s. 58-67
Number of pages	10 s.
Language	eng - English
Country	US - United States
Keywords	Fascioloides magna ; Fasciola hepatica ; ribosomal genes ; mitochondrial genes
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GD524/03/H133 GA ČR - Czech Science Foundation (CSF)
	IAA6022404 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000253731700009
Annotation	Complete sequences of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna are presented and were compared with previously published sequences of the liver fluke Fasciola hepatica. Fixed interspecific genetic differences were determined that allow molecular differentiation of F. magna and F. hepatica using either the PCR-RFLP method or PCR amplification of species-specific DNA regions. Additionally, intraspecific sequence polymorphism of the complete cox1 and nad1 mitochondrial genes in geographically distinct F. magna populations was determined.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2009

Number of the records: 1